Nathaniel Mayo Sr. was born between 1625 and 1627 at England. He was the son of
Rev. John Mayo Sr. and
Tamisen Brike. On 1643 on the list of those able to bear arms for the first time.
Nathaniel Mayo Sr. married
Hannah Prence, daughter of
Gov. Thomas Prence and
Patience Brewster, on 14 February 1648/49 at Eastham, Barnstable County, Massachusetts. Nathaniel Mayo Sr. married
Hannah Prence, daughter of
Gov. Thomas Prence and
Patience Brewster, on 13 February 1649/50 at Eastham, Barnstable County, Massachusetts. Nathaniel Mayo Sr. left a will on 19 December 1661 at Eastham, Barnstable County, Massachusetts. He died in 1661/62 at Eastham, Barnstable County, Massachusetts. His estate was proved on 4 March 1661/62.